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dc.contributor.authorHeras, Sandra
dc.contributor.authorPlanella, Laia
dc.contributor.authorCaldarazzo, Ilaria
dc.contributor.authorVera Rodríguez, Manuel
dc.contributor.authorGarcía Marín, José Luis
dc.contributor.authorRoldán, María Inés
dc.date.accessioned2021-05-04T13:02:48Z
dc.date.available2021-05-04T13:02:48Z
dc.date.issued2016
dc.identifier.citationHeras S, Planella L, Caldarazzo I, Vera M, García-Marín J, Roldán MI. 2016. Development and characterization of novel microsatellite markers by Next Generation Sequencing for the blue and red shrimp Aristeus antennatus. PeerJ 4:e2200 https://doi.org/10.7717/peerj.2200
dc.identifier.urihttp://hdl.handle.net/10347/26111
dc.description.abstractThe blue and red shrimp, Aristeus antennatus, is a commercially important crustacean, in the Mediterranean Sea, which has been listed as a priority species for fishery management. Hypervariable microsatellite markers could be a useful tool to identify genetic stocks among geographically close fishing grounds. Potential microsatellite markers (97) identified from next-generation sequencing of an individual shrimp using a 454 GS Junior Pyrosequencer were tested on a preliminary panel of 15 individuals representing the four worldwide genetic stocks of the species from which 35 polymorphic loci were identified and used to characterize an additional 20 individuals from the Western Mediterranean Sea. In the Western Mediterranean sample, 32 out of 35 were polymorphic loci and the number of alleles per locus ranged from 2 to 14 and expected heterozygosity ranged from 0.050 to 0.968. No linkage disequilibrium was detected, indicating the independence of the loci. These novel microsatellites provide additional tools to address questions relating to genetic diversity, parentage studies and connectivity patterns of A. antennatus populations and help develop effective strategies to ensure long-term sustainability of this resource
dc.description.sponsorshipThis work was supported by grants by the Spanish Government (AGL2009-09228 and CTM2014-54648-C2-2-R) and Ajuntament de Palamós, Spain (Beca Coopoerativa La Equitativa 2013–2014) to MIR. LP benefited from predoctoral fellowship from the Universitat de Girona (BR2014), Spain. IC benefited from a 6 months fellowship from Regione Toscana, Italy, during her stay in Girona
dc.language.isoeng
dc.publisherPeerJ Inc.
dc.rights© 2016 Heras et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited
dc.rightsAtribución 4.0 Internacional
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectAristeus antennatus
dc.subjectNext-generation sequencing
dc.subjectMicrosatellite loci
dc.subjectPopulation genetics
dc.subjectGenetic stocks
dc.subjectBlue and red shrimp
dc.titleDevelopment and characterization of novel microsatellite markers by Next Generation Sequencing for the blue and red shrimp Aristeus antennatus
dc.typeinfo:eu-repo/semantics/article
dc.identifier.DOI10.7717/peerj.2200
dc.relation.publisherversionhttps://doi.org/10.7717/peerj.2200
dc.type.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.e-issn2167-8359
dc.rights.accessrightsinfo:eu-repo/semantics/openAccess
dc.contributor.affiliationUniversidade de Santiago de Compostela. Departamento de Zooloxía, Xenética e Antropoloxía Física
dc.description.peerreviewedSI


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© 2016 Heras et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited
Except where otherwise noted, this item's license is described as  © 2016 Heras et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited





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