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dc.contributor.authorRonza, Paolo
dc.contributor.authorRobledo Sánchez, Diego
dc.contributor.authorBermúdez Pose, Roberto
dc.contributor.authorLosada García, Ana Paula
dc.contributor.authorGómez Pardo, María Belén
dc.contributor.authorSitjà Bobadilla, Ariadna
dc.contributor.authorQuiroga Berdeal, María Isabel
dc.contributor.authorMartínez Portela, Paulino
dc.date.accessioned2021-05-07T12:28:10Z
dc.date.available2021-05-07T12:28:10Z
dc.date.issued2016
dc.identifier.citationInternational Journal for Parasitology 46(8): 507-517 (2016)
dc.identifier.issn1879-0135
dc.identifier.urihttp://hdl.handle.net/10347/26152
dc.description.abstractEnteromyxum scophthalmi, an intestinal myxozoan parasite, is the causative agent of a threatening disease for turbot (Scophthalmus maximus, L.) aquaculture. The colonisation of the digestive tract by this parasite leads to a cachectic syndrome associated with high morbidity and mortality rates. This myxosporidiosis has a long pre-patent period and the first detectable clinical and histopathological changes are subtle. The pathogenic mechanisms acting in the early stages of infection are still far from being fully understood. Further information on the host–parasite interaction is needed to assist in finding efficient preventive and therapeutic measures. Here, a RNA-seq-based transcriptome analysis of head kidney, spleen and pyloric caeca from experimentally-infected and control turbot was performed. Only infected fish with early signs of infection, determined by histopathology and immunohistochemical detection of E. scophthalmi, were selected. The RNA-seq analysis revealed, as expected, less intense transcriptomic changes than those previously found during later stages of the disease. Several genes involved in IFN-related pathways were up-regulated in the three organs, suggesting that the IFN-mediated immune response plays a main role in this phase of the disease. Interestingly, an opposite expression pattern had been found in a previous study on severely infected turbot. In addition, possible strategies for immune system evasion were suggested by the down-regulation of different genes encoding complement components and acute phase proteins. At the site of infection (pyloric caeca), modulation of genes related to different structural proteins was detected and the expression profile indicated the inhibition of cell proliferation and differentiation. These transcriptomic changes provide indications regarding the mechanisms of parasite attachment to and invasion of the host. The current results contribute to a better knowledge of the events that characterise the early stages of turbot enteromyxosis and provide valuable information to identify molecular markers for early detection and control of this important parasitosis
dc.description.sponsorshipThis study was funded by the Spanish Ministry of Economy and Competitiveness (AGL 2009-13282-C02-01 and -02; AGL2015-67039-C3-1-R and AGL2015-67039-C3-3-R), the European Regional Development Fund (ERDF, European Union) and Xunta de Galicia (Spain) local government (GRC2014/010 and GPC2015/34). Diego Robledo was supported by a FPU fellowship funded by the Spanish Ministry of Education, Culture and Sport. Paolo Ronza was supported by a grant from the scientific network “INMUNOGENOM”, funded by Xunta de Galicia (REDES GI-1251)
dc.language.isoeng
dc.publisherElsevier
dc.rights© 2016 Australian Society for Parasitology. Published by Elsevier Ltd. This manuscript version is made available under the CC-BY-NC-ND 4.0 license (http://creativecommons.org/licenses/by-nc-nd/4.0/)
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectRNA-seq
dc.subjectTranscriptome
dc.subjectTurbot
dc.subjectEnteromyxum scophthalmi
dc.subjectMyxozoa
dc.subjectPathogenesis
dc.titleRNA-seq analysis of early enteromyxosis in turbot (Scophthalmus maximus): new insights into parasite invasion and immune evasion strategies
dc.typejournal article
dc.identifier.doi10.1016/j.ijpara.2016.03.007
dc.relation.publisherversionhttps://doi.org/10.1016/j.ijpara.2016.03.007
dc.type.hasVersionAM
dc.rights.accessRightsopen access
dc.contributor.affiliationUniversidade de Santiago de Compostela. Departamento de Anatomía, Produción Animal e Ciencias Clínicas Veterinarias
dc.contributor.affiliationUniversidade de Santiago de Compostela. Departamento de Zooloxía, Xenética e Antropoloxía Física
dc.description.peerreviewedSI
dc.relation.projectIDinfo:eu-repo/grantAgreement/MICINN/Plan Nacional de I+D+i 2008-2011/AGL2009-13282-C02-01/ES/Control De Las Enteromixosis Del Rodaballo Y Esparidos. Nuevas Aproximaciones Basadas En La Caracterizacion Genetica, Antigenica Y Estructural De Los Parasitos Y De La Interaccion Parasito-Hospedador
dc.relation.projectIDinfo:eu-repo/grantAgreement/MICINN/Plan Nacional de I+D+i 2008-2011/AGL2009-13282-C02-02/ES/Control De Las Enteromixosis Del Rodaballo Y Esparidos. Caracterizacion De La Patogenia, La Interaccion Parasito-Hospedador Y La Respuesta Inmunitaria
dc.relation.projectIDinfo:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2015-67039-C3-1-R/ES/BUSQUEDA DE HERRAMIENTAS DE DIAGNOSTICO PRECOZ Y DETECCION DE BIOMARCADORES DE SALUD/ENFERMEDAD INTESTINAL EN RODABALLO: ENTEROMIXOSIS COMO MODELO DE ESTUDIO


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© 2016 Australian Society for Parasitology. Published by Elsevier Ltd. This manuscript version is made available under the CC-BY-NC-ND 4.0 license (http://creativecommons.org/licenses/by-nc-nd/4.0/)
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 © 2016 Australian Society for Parasitology. Published by Elsevier Ltd. This manuscript version is made available under the CC-BY-NC-ND 4.0 license (http://creativecommons.org/licenses/by-nc-nd/4.0/)





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