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dc.contributor.authorHasanuzzaman, Abul Farah Md
dc.contributor.authorCao Hermida, María Asunción
dc.contributor.authorRonza, Paolo
dc.contributor.authorFernández Boo, Sergio
dc.contributor.authorRubiolo Gaytán, Juan Andrés
dc.contributor.authorRobledo Sánchez, Diego
dc.contributor.authorGómez Tato, Antonio
dc.contributor.authorÁlvarez Dios, José Antonio
dc.contributor.authorGómez Pardo, María Belén
dc.contributor.authorVillalba García, Antonio
dc.contributor.authorMartínez Portela, Paulino
dc.date.accessioned2021-05-24T07:11:33Z
dc.date.available2021-05-24T07:11:33Z
dc.date.issued2020
dc.identifier.citationInternational Journal for Parasitology. 50 (2020). 195–208
dc.identifier.issn0020-7519
dc.identifier.urihttp://hdl.handle.net/10347/26266
dc.description.abstractThe Manila clam (Ruditapes philippinarum) is the bivalve species with the highest global production from both fisheries and aquaculture, but its production is seriously threatened by perkinsosis, a disease caused by the protozoan parasite Perkinsus olseni. To understand the molecular mechanisms underlying R. philippinarum–P. olseni interactions, we analysed the gene expression profiles of in vitro challenged clam hemocytes and P. olseni trophozoites, using two oligo-microarray platforms, one previously validated for R. philippinarum hemocytes and a new one developed and validated in this study for P. olseni. Manila clam hemocytes were in vitro challenged with trophozoites, zoospores, and extracellular products from P. olseni in vitro cultures, while P. olseni trophozoites were in vitro challenged with Manila clam plasma along the same time-series (1 h, 8 h, and 24 h). The hemocytes showed a fast activation of the innate immune response, particularly associated with hemocyte recruitment, in the three types of challenges. Nevertheless, different immune-related pathways were activated in response to the different parasite stages, suggesting specific recognition mechanisms. Furthermore, the analyses provided useful complementary data to previous in vivo challenges, and confirmed the potential of some proposed biomarkers. The combined analysis of gene expression in host and parasite identified several processes in both the clam and P. olseni, such as redox and glucose metabolism, protease activity, apoptosis and iron metabolism, whose modulation suggests cross-talk between parasite and host. This information might be critical to determine the outcome of the infection, thus highlighting potential therapeutic targets. Altogether, the results of this study aid understanding the response and interaction between R. philippinarum and P. olseni, and will contribute to developing effective control strategies for this threatening parasitosis
dc.description.sponsorshipThis work was funded by the Ministerio de Economía y Competitividad of the Spanish Government, through the projects (AGL2011-30449-C02-01 and AGL2012-37981), the European Regional Development Funds (FEDER 2007–2013) and the Regional Government of Galicia, Xunta de Galicia, Spain, through the projects ED431C 2018/28 and ED431D 2017/21. The study was also supported by the Centro de Supercomputación de Galicia (CESGA). The first author would like to acknowledge the PhD scholarship awarded by the EXPERTS III Consortium of the European Community Mobility Programme “Erasmus Mundus Action 2, Strand 1” (EMA2). SFB was supported by a scholarship of the Consellería do Mar da Xunta de Galicia
dc.language.isoeng
dc.publisherElsevier
dc.rights© 2020 Published by Elsevier Ltd on behalf of Australian Society for Parasitology. This manuscript version is made available under the CC-BY-NC-ND 4.0 license (http://creativecommons.org/licenses/by-nc-nd/4.0/)
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectManila clam
dc.subjectPerkinsosis
dc.subjectIn vitro challenge
dc.subjectGene expression
dc.subjectHemocytes
dc.subjectHost–parasite interaction
dc.titleNew insights into the Manila clam – Perkinsus olseni interaction based on gene expression analysis of clam hemocytes and parasite trophozoites through in vitro challenges
dc.typejournal article
dc.identifier.doi10.1016/j.ijpara.2019.11.008
dc.relation.publisherversionhttps://doi.org/10.1016/j.ijpara.2019.11.008
dc.type.hasVersionAM
dc.rights.accessRightsopen access
dc.contributor.affiliationUniversidade de Santiago de Compostela. Departamento de Anatomía, Produción Animal e Ciencias Clínicas Veterinarias
dc.contributor.affiliationUniversidade de Santiago de Compostela. Departamento de Matemática Aplicada
dc.contributor.affiliationUniversidade de Santiago de Compostela. Departamento de Matemáticas
dc.contributor.affiliationUniversidade de Santiago de Compostela. Departamento de Zooloxía, Xenética e Antropoloxía Física
dc.contributor.affiliationUniversidade de Santiago de Compostela. Instituto de Acuicultura
dc.description.peerreviewedSI
dc.relation.projectIDinfo:eu-repo/grantAgreement/MICINN/Plan Nacional de I+D+i 2008-2011/AGL2011-30449-C02-01/ES/PERKINSOSIS DE LA ALMEJA: EXPRESION PROTEINICA EN LOS HEMOCITOS Y PLASMA DEL HOSPEDADOR Y EN EL PARASITO DURANTE SU INTERACCION
dc.relation.projectIDinfo:eu-repo/grantAgreement/MINECO/Plan Nacional de I+D+i 2008-2011/AGL2012-37981/ES/ANALISIS DE LA INTERACCION ENTRE HEMOCITOS DE ALMEJA RUDITAPES PHILIPPINARUM Y EL PARASITO PERKINSUS OLSENI MEDIANTE ESTUDIO DE LA EXPRESION GENICA CON MICROARRAYS


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 © 2020 Published by Elsevier Ltd on behalf of Australian Society for Parasitology. This manuscript version is made available under the CC-BY-NC-ND 4.0 license (http://creativecommons.org/licenses/by-nc-nd/4.0/)





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