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dc.contributor.authorPazo Pascual, Marta
dc.contributor.authorSalluce, Giulia
dc.contributor.authorLostalé Seijo, Irene
dc.contributor.authorJuanes Carrasco, María Luisa
dc.contributor.authorGonzález García, Francisco
dc.contributor.authorGarcía Fandiño, Rebeca
dc.contributor.authorMontenegro García, Javier
dc.date.accessioned2021-09-06T16:11:51Z
dc.date.available2021-09-06T16:11:51Z
dc.date.issued2021
dc.identifier.citationRSC Chem. Biol., 2021, 2, 503-512
dc.identifier.urihttp://hdl.handle.net/10347/26917
dc.description.abstractIn this work we report a rational design strategy for the identification of new peptide prototypes for the non-disruptive supramolecular permeation of membranes and the transport of different macromolecular giant cargos. The approach targets a maximal enhancement of helicity in the presence of membranes with sequences bearing the minimal number of cationic and hydrophobic moieties. The here reported folding enhancement in membranes allowed the selective non-lytic translocation of different macromolecular cargos including giant proteins. The transport of different high molecular weight polymers and functional proteins was demonstrated in vesicles and in cells with excellent efficiency and optimal viability. As a proof of concept, functional monoclonal antibodies were transported for the first time into different cell lines and cornea tissues by exploiting the helical control of a short peptide sequence. This work introduces a rational design strategy that can be employed to minimize the number of charges and hydrophobic residues of short peptide carriers to achieve non-destructive transient membrane permeation and transport of different macromolecules
dc.description.sponsorshipThis work was partially supported by the Spanish Agencia Estatal de Investigación (AEI) [SAF2017-89890-R], the Xunta de Galicia (ED431C 2017/25, 2016-AD031 and Centro Singular de Investigación de Galicia accreditation 2019–2022, ED431G 2019/03), the European Union (European Regional Development Fund – ERDF) and the ISCIII (RD16/0008/003). M. P. thanks the Xunta de Galicia (ED481A-2017/142), and M. J. and G. S. thank MINECO for their F. P. I. fellowships (BES-2015-071779; PRE2018-085973). R. G.-F. thanks a RyC (RYC-2016-20335), MINECO (RTI2018-098795-A-I00) and Xunta de Galicia (ED431F 2020/05). J. M. received a RyC (RYC-2013-13784), an ERC-Stg (DYNAP-677786), a HFSP (RGY0066/2017) and ISCIII (COV20/00297). All calculations were carried out at Centro de Supercomputación de Galicia (CESGA)
dc.language.isoeng
dc.publisherRoyal Society of Chemistry
dc.relationinfo:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BES-2015-071779/ES
dc.relationinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PRE2018-085973/ES
dc.relationinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/SAF2017-89890-R/ES/PEPTIDOS HIBRIDOS PARA EL TRANSPORTE SELECTIVO Y ENTREGA DE PROTEINAS TERAPEUTICAS
dc.relationinfo:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/RYC-2016-20335/ES
dc.relationinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-098795-A-I00/ES/DISEÑO DE AGENTES ANTITUMORALES A PARTIR DE SIMULACIONES DE DINAMICA MOLECULAR, ANALISIS BIG DATA E INTELIGENCIA ARTIFICIAL VALIDADOS POR EXPERIMENTOS BIOFISICOS
dc.relationinfo:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/RYC-2013-13784/ES
dc.relationinfo:eu-repo/grantAgreement/EC/H2020/677786
dc.rights© 2021 The Author(s). Published by the Royal Society of Chemistry. Open Access Article. This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence
dc.rights.urihttp://creativecommons.org/licenses/by-nc/3.0/
dc.titleShort oligoalanine helical peptides for supramolecular nanopore assembly and protein cytosolic delivery
dc.typeinfo:eu-repo/semantics/article
dc.identifier.DOI10.1039/D0CB00103A
dc.relation.publisherversionhttps://doi.org/10.1039/D0CB00103A
dc.type.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.e-issn2633-0679
dc.rights.accessrightsinfo:eu-repo/semantics/openAccess
dc.contributor.affiliationUniversidade de Santiago de Compostela. Centro de Investigación en Medicina Molecular e Enfermidades Crónicas
dc.contributor.affiliationUniversidade de Santiago de Compostela. Centro de Investigación en Química Biolóxica e Materiais Moleculares
dc.contributor.affiliationUniversidade de Santiago de Compostela. Departamento de Química Orgánica
dc.description.peerreviewedSI


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© 2021 The Author(s). Published by the Royal Society of Chemistry. Open Access Article. This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence
Except where otherwise noted, this item's license is described as  © 2021 The Author(s). Published by the Royal Society of Chemistry. Open Access Article. This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence





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