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dc.contributor.authorWilson, Estibali Wilkie
dc.contributor.authorCastro Bustelo, Verónica
dc.contributor.authorChaves, Raquel
dc.contributor.authorEspinosa, Miguel
dc.contributor.authorRodil Rodríguez, María del Rosario
dc.contributor.authorQuintana Álvarez, José Benito
dc.contributor.authorVieira, María Natividade
dc.contributor.authorSantos, Miguel M.
dc.identifier.citationE.W. Wilson et al. Chemosphere, 2021, 272, 129823
dc.description.abstractSeveral studies show that many water bodies in developing countries are increasingly affected by anthropogenic pressure, such as agricultural activities, domestic and industrial wastewater. However, data is scarce in several of such countries, including Panama. Thus, in this work, the ecotoxicological status of selected rivers in Panama with distinct input sources were evaluated using the zebrafish (Danio rerio) embryo bioassays combined with a liquid chromatography-high resolution mass spectrometry screening of contaminants of emerging concern (CECs), using a library of over 3200 chemicals. A total of 68 CECs, including pharmaceuticals and metabolites, pesticides and several industrial chemicals, could be tentatively identified. Additionally, the zebrafish embryo bioassays showed a significant increase (p < 0.05) in embryo mortality/abnormalities when incubated with water samples from two rivers, Matasnillo and Curundú (47.5% and 32%, respectively). Importantly, a positive correlation between ecotoxicological endpoints and some of the detected CECs was observed. The findings demonstrate that both rivers are under strong anthropogenic pressure, and therefore, management actions are urgently needed to decrease their level of contamination. Overall, this study further supports the use of the zebrafish embryo bioassay as a fast, high throughput approach for screening the toxicity of water samples, and highlights the advantages of combining ecotoxicological assays with high-resolution mass spectrometry to an expedite assessment of the ecotoxicological status of water bodies.
dc.description.sponsorshipThis work was supported by the Portuguese Foundation for Science and Technology (FCT) (PTDC|CTA-AMB|31554|2017; UIDB/04423/2020; UIDP/04423/2020), the Spanish Agencia Estatal de Investigación (ref. CTM2017-84763-C3-2-R), the Galician Council of Culture, Education and Universities (ref. ED431C2017/36 and Verónica Castro predoctoral contract, ref. ED481A-2017/156), co-funded by the European Regional Development Fund (ERDF/FEDER).
dc.relationinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/CTM2017-84763-C3-2-R/ES/EVALUACION DEL EXPOSOMA DE CONTAMINANTES EMERGENTES EN AMBIENTES ACUATICOS
dc.rights© 2021, Elsevier. This manuscript version is made available under the CC-BY-NC-ND 4.0 license (
dc.subjectEmerging contaminants
dc.subjectQuadrupole-time-of flight mass spectrometry (QTOF)
dc.subjectRisk assessment
dc.subjectToxicity testing
dc.subjectDanio rerio
dc.titleUsing zebrafish embryo bioassays combined with high-resolution mass spectrometry screening to assess ecotoxicological water bodies quality status: A case study in Panama rivers
dc.contributor.affiliationUniversidade de Santiago de Compostela. Departamento de Química Analítica, Nutrición e Bromatoloxía
dc.contributor.affiliationUniversidade de Santiago de Compostela. Instituto de Investigación e Análises Alimentarias

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© 2021, Elsevier. This manuscript version is made available under the CC-BY-NC-ND 4.0 license (
Except where otherwise noted, this item's license is described as  © 2021, Elsevier. This manuscript version is made available under the CC-BY-NC-ND 4.0 license (

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