Approaches to liquid chromatography tandem mass spectrometry assessment of glyphosate residues in wine
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Título: | Approaches to liquid chromatography tandem mass spectrometry assessment of glyphosate residues in wine
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Autor/a: | Pérez Mayán, Leticia
Castro Varela, Gabriela
Ramil Criado, María
Cela Torrijos, Rafael
Rodríguez Pereiro, Isaac
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Centro/Departamento: | Universidade de Santiago de Compostela. Departamento de Química Analítica, Nutrición e Bromatoloxía Universidade de Santiago de Compostela. Instituto de Investigación en Análises Químicas e Biolóxicas
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Palabras chave: | Glyphosate | Wine | Liquid chromatography tandem mass spectrometry | Derivatization | Direct injection | |
Data: | 2021
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Editor: | Springer
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Cita bibliográfica: | Pérez-Mayán, L., Castro, G., Ramil, M. et al. Approaches to liquid chromatography tandem mass spectrometry assessment of glyphosate residues in wine. Anal Bioanal Chem (2021). https://doi.org/10.1007/s00216-021-03775-w
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Resumo: | The performance of two different analytical methodologies to investigate the presence of glyphosate (GLY) and aminomethylphosphonic acid (AMPA) residues in wine samples was evaluated. Transformation of compounds in their fluorene-9-methyloxycarbonyl derivatives permitted their separation under reversed-phase liquid chromatography with tandem mass spectrometry (LC–MS/MS) determination. Although the wine matrix severely impaired the efficiency of GLY derivatization, this drawback was solved using a molecularly imprinted sorbent for the previous, selective extraction of GLY and AMPA from wine. Alternatively, the use of a strong anionic exchange, polyvinyl alcohol-based LC column, turned to be the most effective alternative for direct determination of both compounds in diluted wine samples. The chromatographic behavior of this column and the magnitude of matrix effects observed during analysis of diluted wine samples were significantly affected by the composition of the mobile phase. Under final working conditions, this column permitted the separation of AMPA and the fungicide fosetyl (which shows common transitions in tandem MS/MS methods), it improved significantly the sample throughput versus extraction-derivatization-purification method, and it allowed the use of solvent-based calibration standards. Both analytical procedures provided similar limits of quantification (LOQs) for GLY (0.5–1.0 ng mL−1), while the multistep method was 8 times more sensitive to AMPA than the direct procedure. GLY residues stayed above method LOQs in 70% of the processed wines; however, concentrations measured in 95% of positive samples remained 100 times below the maximum residue limit (MRL) set for GLY in vinification grapes |
Versión do editor: | https://doi.org/10.1007/s00216-021-03775-w |
URI: | http://hdl.handle.net/10347/27293
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DOI: | 10.1007/s00216-021-03775-w |
ISSN: | 1618-2642
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E-ISSN: | 1618-2650
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Dereitos: | © The Author(s) 2021. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ Atribución 4.0 Internacional
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