Supercritical CO2 technology for one-pot foaming and sterilization of polymeric scaffolds for bone regeneration
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Title: | Supercritical CO2 technology for one-pot foaming and sterilization of polymeric scaffolds for bone regeneration |
Author: | Santos Rosales, Víctor Magariños Ferro, Beatriz Starbird-Perez, Ricardo Fariña Espinosa, José Bruno Celso Álvarez Lorenzo, Carmen Isabel García-González, Carlos A |
Affiliation: | Universidade de Santiago de Compostela. Departamento de Farmacoloxía, Farmacia e Tecnoloxía Farmacéutica Universidade de Santiago de Compostela. Departamento de Microbioloxía e Parasitoloxía |
Subject: | Sterilization | Spores | Supercritical CO2 | Hydrogen peroxide | Bone scaffold | |
Date of Issue: | 2021 |
Publisher: | Elsevier |
Citation: | International Journal of Pharmaceutics 605 (2021) 120801 |
Abstract: | Sterilization is a quite challenging step in the development of novel polymeric scaffolds for regenerative medicine since conventional sterilization techniques may significantly alter their morphological and physicochemical properties. Supercritical (sc) sterilization, i.e. the use of scCO2 as a sterilizing agent, emerges as a promising sterilization method due to the mild operational conditions and excellent penetration capability. In this work, a scCO2 protocol was implemented for the one-pot preparation and sterilization of poly(-caprolactone) (PCL)/poly(lactic-co-glycolic acid) (PLGA) scaffolds. The sterilization conditions were established after screening against both Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli, Pseudomonas aeruginosa) vegetative bacteria and spores of Bacillus stearothermophilus, Bacillus pumilus and Bacillus atrophaeus. The transition from the sterilization conditions (140 bar, 39 °C) to the compressed foaming (60 bar, 26 °C) was performed through controlled depressurization (3.2 bar/min) and CO2 liquid flow. Controlled depressurization/pressurization cycles were subsequently applied. Using this scCO2 technology toolbox, sterile scaffolds of well-controlled pore architecture were obtained. This sterilization procedure successfully achieved not only SAL-6 against well-known resistant bacteria endospores but also improved the scaffold morphologies compared to standard gamma radiation sterilization procedures |
Publisher version: | https://doi.org/10.1016/j.ijpharm.2021.120801 |
URI: | http://hdl.handle.net/10347/27352 |
DOI: | 10.1016/j.ijpharm.2021.120801 |
E-ISSN: | 0378-5173 |
Rights: | © 2021 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/) Atribución 4.0 Internacional |
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