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dc.contributor.authorSantos Rosales, Víctor
dc.contributor.authorMagariños Ferro, Beatriz
dc.contributor.authorStarbird-Perez, Ricardo
dc.contributor.authorFariña Espinosa, José Bruno Celso
dc.contributor.authorÁlvarez Lorenzo, Carmen Isabel
dc.contributor.authorGarcía-González, Carlos A
dc.identifier.citationInternational Journal of Pharmaceutics 605 (2021) 120801
dc.description.abstractSterilization is a quite challenging step in the development of novel polymeric scaffolds for regenerative medicine since conventional sterilization techniques may significantly alter their morphological and physicochemical properties. Supercritical (sc) sterilization, i.e. the use of scCO2 as a sterilizing agent, emerges as a promising sterilization method due to the mild operational conditions and excellent penetration capability. In this work, a scCO2 protocol was implemented for the one-pot preparation and sterilization of poly(-caprolactone) (PCL)/poly(lactic-co-glycolic acid) (PLGA) scaffolds. The sterilization conditions were established after screening against both Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli, Pseudomonas aeruginosa) vegetative bacteria and spores of Bacillus stearothermophilus, Bacillus pumilus and Bacillus atrophaeus. The transition from the sterilization conditions (140 bar, 39 °C) to the compressed foaming (60 bar, 26 °C) was performed through controlled depressurization (3.2 bar/min) and CO2 liquid flow. Controlled depressurization/pressurization cycles were subsequently applied. Using this scCO2 technology toolbox, sterile scaffolds of well-controlled pore architecture were obtained. This sterilization procedure successfully achieved not only SAL-6 against well-known resistant bacteria endospores but also improved the scaffold morphologies compared to standard gamma radiation sterilization procedures
dc.description.sponsorshipThis work was supported by Xunta de Galicia [ED431F 2016/01, ED431C 2020/17], MCIUN [RTI2018-094131-A-I00], MINECO [SAF2017-83118-R], Consellería de Sanidade, Servizo Galego de Saúde, Axencia de Coñecemento e Saúde (ACIS, CT850A-G), Agencia Estatal de Investigación [AEI] and FEDER funds. V. Santos-Rosales acknowledges to Xunta de Galicia (Consellería de Cultura, Educación e Ordenación Universitaria) for a predoctoral research fellowship [ED481A-2018/014]. C.A. García-González acknowledges to MINECO for a Ramón y Cajal Fellowship [RYC2014-15239]
dc.rights© 2021 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY license (
dc.rightsAtribución 4.0 Internacional
dc.subjectSupercritical CO2
dc.subjectHydrogen peroxide
dc.subjectBone scaffold
dc.titleSupercritical CO2 technology for one-pot foaming and sterilization of polymeric scaffolds for bone regeneration
dc.contributor.affiliationUniversidade de Santiago de Compostela. Departamento de Farmacoloxía, Farmacia e Tecnoloxía Farmacéutica
dc.contributor.affiliationUniversidade de Santiago de Compostela. Departamento de Microbioloxía e Parasitoloxía

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© 2021 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY license (
Except where otherwise noted, this item's license is described as  © 2021 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY license (

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