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dc.contributor.authorIllodo, Sara
dc.contributor.authorPérez González, Cibrán
dc.contributor.authorBarcia, Ramiro
dc.contributor.authorRodriguez-Prieto, Flor
dc.contributor.authorAl-Soufi, Wajih
dc.contributor.authorNovo Rodríguez, Mercedes
dc.date.accessioned2022-01-20T09:20:40Z
dc.date.available2022-01-20T09:20:40Z
dc.date.issued2022
dc.identifier.citationIllodo, S.; Pérez-González, C.; Barcia, R.; Rodríguez-Prieto, F.; Al-Soufi, W.; Novo, M. Spectroscopic Characterization of Mitochondrial G-Quadruplexes. Int. J. Mol. Sci. 2022, 23, 925. https://doi.org/10.3390/ ijms23020925
dc.identifier.issn1422-0067
dc.identifier.urihttp://hdl.handle.net/10347/27375
dc.description.abstractGuanine quadruplexes (G4s) are highly polymorphic four-stranded structures formed within guanine-rich DNA and RNA sequences that play a crucial role in biological processes. The recent discovery of the first G4 structures within mitochondrial DNA has led to a small revolution in the field. In particular, the G-rich conserved sequence block II (CSB II) can form different types of G4s that are thought to play a crucial role in replication. In this study, we decipher the most relevant G4 structures that can be formed within CSB II: RNA G4 at the RNA transcript, DNA G4 within the non-transcribed strand and DNA:RNA hybrid between the RNA transcript and the non-transcribed strand. We show that the more abundant, but unexplored, G6AG7 (37%) and G6AG8 (35%) sequences in CSB II yield more stable G4s than the less profuse G5AG7 sequence. Moreover, the existence of a guanine located 1 bp upstream promotes G4 formation. In all cases, parallel G4s are formed, but their topology changes from a less ordered to a highly ordered G4 when adding small amounts of potassium or sodium cations. Circular dichroism was used due to discriminate different conformations and topologies of nucleic acids and was complemented with gel electrophoresis and fluorescence spectroscopy studies
dc.description.sponsorshipThis research was funded by the Gobierno de España—Ministerio de Economía y Competitividad, grant number CTQ2014-59020-R, the Xunta de Galicia, grant numbers ED431B 2019/18, ED431G 2019/03 Centro singular de investigación de Galicia accreditation 2019–2022, and Ph.D. fellowship to S.I. and the European Union (European Regional Development Fund—ERDF)
dc.language.isoeng
dc.publisherMDPI
dc.rights© 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/)
dc.rightsAtribución 4.0 Internacional
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectDNA quadruplexes
dc.subjectG-quadruplex CD and fluorescence spectroscopy
dc.subjectG-quadruplex structure
dc.titleSpectroscopic characterization of mitochondrial G-quadruplexes
dc.typeinfo:eu-repo/semantics/article
dc.identifier.DOI10.3390/ijms23020925
dc.relation.publisherversionhttps://doi.org/10.3390/ijms23020925
dc.type.versioninfo:eu-repo/semantics/publishedVersion
dc.rights.accessrightsinfo:eu-repo/semantics/openAccess
dc.contributor.affiliationUniversidade de Santiago de Compostela. Centro de Investigación en Química Biolóxica e Materiais Moleculares
dc.contributor.affiliationUniversidade de Santiago de Compostela. Departamento de Bioquímica e Bioloxía Molecular
dc.contributor.affiliationUniversidade de Santiago de Compostela. Departamento de Química Física
dc.description.peerreviewedSI


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© 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/)
Except where otherwise noted, this item's license is described as  © 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/)





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