Canonical and novel non-canonical activities of the Holliday junction resolvase Yen1
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Título: | Canonical and novel non-canonical activities of the Holliday junction resolvase Yen1
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Autor/a: | Carreira Rodríguez, Raquel
Aguado Domínguez, Francisco Javier
Hurtado Nieves, Vanesa
González Blanco, Miguel
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Centro/Departamento: | Universidade de Santiago de Compostela. Centro de Investigación en Química Biolóxica e Materiais Moleculares Universidade de Santiago de Compostela. Departamento de Bioquímica e Bioloxía Molecular
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Data: | 2022
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Editor: | Oxford University Press
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Cita bibliográfica: | Nucleic Acids Research, Volume 50, Issue 1, 11 January 2022, Pages 259–280, https://doi.org/10.1093/nar/gkab1225
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Resumo: | Yen1 and GEN1 are members of the Rad2/XPG family of nucleases that were identified as the first canonical nuclear Holliday junction (HJ) resolvases in budding yeast and humans due to their ability to introduce two symmetric, coordinated incisions on opposite strands of the HJ, yielding nicked DNA products that could be readily ligated. While GEN1 has been extensively characterized in vitro, much less is known about the biochemistry of Yen1. Here, we have performed the first in-depth characterization of purified Yen1. We confirmed that Yen1 resembles GEN1 in many aspects, including range of substrates targeted, position of most incisions they produce or the increase in the first incision rate by assembly of a dimer on a HJ, despite minor differences. However, we demonstrate that Yen1 is endowed with additional nuclease activities, like a nick-specific 5′-3′ exonuclease or HJ arm-chopping that could apparently blur its classification as a canonical HJ resolvase. Despite this, we show that Yen1 fulfils the requirements of a canonical HJ resolvase and hypothesize that its wider array of nuclease activities might contribute to its function in the removal of persistent recombination or replication intermediates |
Versión do editor: | https://doi.org/10.1093/nar/gkab1225 |
URI: | http://hdl.handle.net/10347/28889
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DOI: | 10.1093/nar/gkab1225 |
ISSN: | 0305-1048
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E-ISSN: | 1362-4962
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Dereitos: | © The Author(s) 2021. Published by Oxford University Press on behalf of Nucleic Acids Research. This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com Atribución-NoComercial 4.0 Internacional
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