Browsing Bioquímica e Bioloxía Molecular by Issue Date
Now showing items 1-20 of 65
-
Conservation of the S10-spc-alpha Locus within Otherwise Highly Plastic Genomes Provides Phylogenetic Insight into the Genus Leptospira
(PLOS, 2008)S10-spc-α is a 17.5 kb cluster of 32 genes encoding ribosomal proteins. This locus has an unusual composition and organization in Leptospira interrogans. We demonstrate the highly conserved nature of this region among ... -
CTLs are targeted to kill β cells in patients with type 1 diabetes through recognition of a glucose-regulated preproinsulin epitope
(American Society for Clinical Investigation, 2008)The final pathway of β cell destruction leading to insulin deficiency, hyperglycemia, and clinical type 1 diabetes is unknown. Here we show that circulating CTLs can kill β cells via recognition of a glucose-regulated ... -
Soluble CD26 Levels and Its Association to Epidemiologic Parameters in a Sample Population
(Hindawi, 2009)Introduction: Previous studies have suggested the use of soluble CD26 (sCD26) as a tumour marker for the detection of colorectal cancer (CRC) and advanced adenomas. The aim of this study was to assess the sCD26 concentration ... -
Serum CD26 is related to histopathological polyp traits and behaves as a marker for colorectal cancer and advanced adenomas
(BMC, 2010)Background: Serum CD26 (sCD26) levels were previously found diminished in colorectal cancer (CRC) patients compared to healthy donors, suggesting its potential utility for early diagnosis. Therefore we aimed to estimate ... -
IC-Tagging and Protein Relocation to ARV muNS Inclusions: A Method to Study Protein-Protein Interactions in the Cytoplasm or Nucleus of Living Cells
(PLOS, 2010)Background: Characterization of protein-protein interactions is essential for understanding cellular functions. Although there are many published methods to analyze protein-protein interactions, most of them present serious ... -
A Versatile Molecular Tagging Method for Targeting Proteins to Avian Reovirus muNS Inclusions. Use in Protein Immobilization and Purification
(PLOS, 2010)Background: Avian reoviruses replicate in viral factories, which are dense cytoplasmic compartments estabilished by protein-protein interactions. The non-structural protein muNS forms the factory scaffold that attracts ... -
Differential inhibitor sensitivity between human kinases VRK1 and VRK2
(PLOS, 2011)Human vaccinia-related kinases (VRK1 and VRK2) are atypical active Ser-Thr kinases implicated in control of cell cycle entry, apoptosis and autophagy, and affect signalling by mitogen activated protein kinases (MAPK). The ... -
Serum activity of DPPIV and its expression on lymphocytes in patients with melanoma and in people with vitiligo
(BMC, 2012)Background: Dipeptidyl peptidase IV, a multifunctional serine protease, is implicated in regulation of malignant transformation, promotion and further progression of cancer, exerting tumor-suppressing or even completely opposite ... -
Virtual Reality as a Tool for Evaluation of Repetitive Rhythmic Movements in the Elderly and Parkinson's Disease Patients
(PLOS, 2012)This work presents an immersive Virtual Reality (VR) system to evaluate, and potentially treat, the alterations in rhythmic hand movements seen in Parkinson's disease (PD) and the elderly (EC), by comparison with healthy ... -
Different intracellular distribution of avian reovirus core protein sigmaA in cells of avian and mammalian origin
(Elsevier, 2012)A comparative analysis of the intracellular distribution of avian reovirus (ARV) core protein sigmaA in cells of avian and mammalian origin revealed that, whereas the viral protein accumulates in the cytoplasm and nucleolus ... -
In Vivo Light-Driven DNA Binding and Cellular Uptake of Nucleic Acid Stains
(American Chemical Society, 2012)Chemical derivatization of nucleic stains such as ethidium bromide or DAPI with tailored, photoresponsive caging groups, allows for “on demand” spatiotemporal control of their in vivo nucleic acid binding, as well as for ... -
Mycobacterium tuberculosis Shikimate Kinase Inhibitors: Design and Simulation Studies of the Catalytic Turnover
(American Chemical Society, 2013)Shikimate kinase (SK) is an essential enzyme in several pathogenic bacteria and does not have any counterpart in human cells, thus making it an attractive target for the development of new antibiotics. The key interactions ... -
Mechanistic Basis of the Inhibition of Type II Dehydroquinase by (2S)- and (2R)-2-Benzyl-3-dehydroquinic Acids
(American Chemical Society, 2013)The structural changes caused by the substitution of the aromatic moiety in (2S)-2-benzyl-3-dehydroquinic acids and its epimers in C2 by electron-withdrawing or electron-donating groups in type II dehydroquinase enzyme ... -
Effects of eslicarbazepine acetate on acute and chronic latrunculin A-induced seizures and extracellular amino acid levels in the mouse hippocampus
(BMC, 2014)Background: Latrunculin A microperfusion of the hippocampus induces acute epileptic seizures and long-term biochemical changes leading to spontaneous seizures. This study tested the effect of eslicarbazepine acetate ... -
Postoperative serum levels of sCD26 for surveillance in colorectal cancer patients
(PLOS, 2014)One of the main aims of the follow-up after curative resection of colorectal cancer is the early detection and treatment of tumor recurrence. We previously demonstrated decreased preoperative soluble CD26 (sCD26) levels ... -
MitoBlue: A Nontoxic and Photostable Blue-Emitting Dye That Selectively Labels Functional Mitochondria
(American Chemical Society, 2014)We report the discovery of a fluorogenic dye, N1,N3-di(2-aminidonaphthalen-6-yl) propane-1,3-diamine, MitoBlue, which selectively stains functional mitochondria while displaying low toxicity, bright blue emission, and high ... -
Exploring the Water-Binding Pocket of the Type II Dehydroquinase Enzyme in the Structure-Based Design of Inhibitors
(American Chemical Society, 2014)Structural and computational studies to explore the WAT1 binding pocket in the structure-based design of inhibitors against the type II dehydroquinase (DHQ2) enzyme are reported. The crystal structures of DHQ2 from M. ... -
Programmed stereoselective assembly of DNA-binding helical metallopeptides
(Royal Society of Chemistry, 2014)A flexible and versatile synthetic approach for the construction of water-stable DNA-binding chiral peptide helicates based on the solid phase peptide synthesis (SPPS) methodology is reported -
Mechanistic insight into the reaction catalysed by bacterial type II dehydroquinases
(Portland Press, 2014-03-15)DHQ2 (type II dehydroquinase), which is an essential enzyme in Helicobacter pylori and Mycobacterium tuberculosis and does not have any counterpart in humans, is recognized to be an attractive target for the development ...