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dc.contributor.authorBoquete Seoane, María Teresa
dc.contributor.authorSchmid, Marc W
dc.contributor.authorWagemaker, niels
dc.contributor.authorCarey, Sarah B.
dc.contributor.authorMcDaniel, Stuart
dc.contributor.authorRichards, Christina
dc.contributor.authorAlonso, Conchita
dc.date.accessioned2022-08-23T11:16:29Z
dc.date.available2022-08-23T11:16:29Z
dc.date.issued2022
dc.identifier.citationEnvironmental and Experimental Botany 201 (2022) 104970
dc.identifier.urihttp://hdl.handle.net/10347/29123
dc.description.abstractThe remarkable capacity of bryophytes to tolerate extremely challenging abiotic conditions allows us to enhance our understanding of the diversity of molecular mechanisms involved in plant stress response. Here, we used next generation sequencing to study DNA methylation and gene expression changes in plants from four populations of the metallophyte moss Scopelophila cataractae experimentally exposed to either Cd or Cu. These populations previously showed differences in tolerance to both metals, so here, we aimed to investigate the molecular basis of this phenotypic differentiation. We found no evidence of genetic differentiation among the populations studied. The epigenetic data, however, showed limited but significant population-specific changes in DNA methylation in response to both metals. Exposure to acute Cu stress in the laboratory led to the downregulation of genes involved in heavy metal tolerance in both the more and the less tolerant populations, but this response was quantitatively higher in the most tolerant. We propose that chronic exposure to varying levels of heavy metals in the field led to potentially non-genetically-based intraspecific differentiation for heavy metal tolerance in S. cataractae. The most tolerant plants invested more in constitutive protection and were more efficient at entering a conservative state when faced with acute Cu stress
dc.description.sponsorshipThis research has received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement No 704141-BryOmics. Dr. M. Teresa Boquete has been supported during the development of this project by the Juan de la Cierva-Incorporación program from the Spanish Ministry of Science, Innovation and Universities (IJC2018-035018) and is currently supported by the Maria Zambrano program from the Spanish Ministry of Science, Innovation and Universities
dc.language.isoeng
dc.publisherElsevier
dc.rights© 2022 The Author(s). Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by- nc-nd/4.0/)
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectAbiotic stress response
dc.subjectDNA methylation
dc.subjectEpigenetics
dc.subjectMetallophyte moss
dc.subjectPhenotypic variation
dc.subjectReduced representation bisulfite sequencing
dc.subjectRNA sequencing
dc.titleMolecular basis of intraspecific differentiation for heavy metal tolerance in the copper moss Scopelophila cataractae
dc.typejournal article
dc.identifier.doi10.1016/j.envexpbot.2022.104970
dc.relation.publisherversionhttps://doi.org/10.1016/j.envexpbot.2022.104970
dc.type.hasVersionVoR
dc.identifier.essn0098-8472
dc.rights.accessRightsopen access
dc.contributor.affiliationUniversidade de Santiago de Compostela. Departamento de Bioloxía Funcional
dc.description.peerreviewedSI


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© 2022 The Author(s). Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by- nc-nd/4.0/)
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 © 2022 The Author(s). Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by- nc-nd/4.0/)





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